Coding

Part:BBa_K3196099:Design

Designed by: Hao Qiu   Group: iGEM19_HUST-China   (2019-10-16)


α-factor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 34
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 34
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
    Illegal XhoI site found at 255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 34
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 34
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

signal peptides

Source

signal peptides

References

邹晨伟,黄义德.毕赤酵母引导外源蛋白分泌的信号肽研究进展[J].生物技术通讯,2020,31(06):781-785.

吴静雯. α-factor信号肽及其N-糖基化修饰对毕赤酵母外源蛋白分泌的影响[D].福建师范大学,2019.